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Bio3:
Lab Final Study Guide - Fall 2006
Exam Format:
There will be a
total of 5 questions, each based on a different lab exercise (some questions
will have multiple parts). You will
be able to use your lab manual and notes during the exam.
Each group member must participate in answering the exam questions,
however, the group may choose to either work on all the questions together, or
split up the work so that one or two people focus on answering a particular
question. Each group may only
consult among themselves in answering the exam questions.
Collaboration among different groups will NOT be allowed.
You will have approximately 2 hours to complete the exam.
Topics:
Lab 1: Process of
Scientific Inquiry
- Be able to design an experiment to answer a biological question.
Be able to outline your experiment by stating the hypothesis, procedure,
results and conclusion.
Lab Appendix A: Tools for Scientific
Inquiry
- Be able to make measurements of weight (mass), volume and length, using
metric units.
- Be able to convert measurements from one unit to another (e.g. meter to
centimeter, Celsius to Fahrenheit)
Lab 2: pH and
Buffers
- Be able to determine whether a solution is an acid or a base based on its
pH value.
- Be able to use red cabbage extract as an indicator solution to measure the pH
of an unknown solution
Lab 3:
Macromolecules
- Be able to determine whether an unknown substance contains a
monosaccharide, starch, protein or fat by using indicators such as Benedict’s
reagent, iodine reagent, biuret reagent and the paper test.
Lab 4: Microscope
- Be able to differentiate between a compound and dissecting microscope.
- Be able to determine the total magnification of an object observed under low
and medium magnification.
- Be able to prepare a slide using a living specimen and draw the image you
observe under the microscope.
Lab 5: Enzymes
- Be able to determine whether a mixture contains the necessary substrate
and enzyme in order for the reaction to proceed, by observing the color change
in the test tube (e.g. the catechol and catecholase experiment)
Lab 6: Respiration
- Be able to design an experiment to compare fermentation rates in solutions
containing different amounts of yeast solution.
- Be able to design an experiment to compare aerobic respiration rates in
solutions containing different amounts of substrate.
Lab 7:
Photosynthesis
- Be able to design an experiment to determine how light intensity or light
color affects the rate of photosynthesis in spinach.
Lab 14: Lab
Digestion
- Be able to determine whether digestion of a protein has occurred by using
the gelatin test, whether digestion of a lipid has occurred by using
phenolphthalein as a pH indicator, and whether digestion of starch has occurred
by using the iodine reagent.
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